Protective effect of Vitamin C and E on enzymatic and antioxidant system in liver and kidney toxicity of Cadmium in rats

Abstract

The present study aimed to evaluate Heavy metals are persistent and uninterrupted ecological pollutants that are capable to cause numerous dysfunctions in target tissues of exposed animals as well as humans. In the present experimental study, 60 male wistar rats were divided into control and experimental groups. CdCl2 + Vitamin C through water + Vitamin E intraperitonially at a dose of mg/Kg B.W/day for 45 days. Finally, Tissue Respiration (TR), Glycolytic, Oxidative, Antioxidant Enzymes and Enzymes and Products measurements. The data were analyzed using SPSS through one way ANOVA test at P < 0.05). In the present study an attempt was made to monitor the impact of Cd-toxicity in the tissues of wistar rats. Enzymes of glycolysis i.e. Phosphorylase ‘a’ and Aldolase were found to significantly be elevated, whereas Kreb's cycle and oxidative enzymes, including SDH and Cytochrome-c-oxidase were found to be significantly inhibited, suggesting the metabolic shift from aerobiosis to anaerobiosis, visualized through increased lactate production at the cellular level. Similarly antioxidant enzymes were also affected during Cd-toxicity, and stimulates the production of ROS, diminishing their structures, prohibition of the roles of antioxidants. The findings from this study showed that a combination of Vitamin C&E enhances the biological recovery induced by Cd, pretreatment with antioxidants including Vitamin C&E decreases the oxidative stress, inhibits progressive fluctuations convinced through Cd, repaired the biochemical alterations and reverted to almost normal metabolic regimes. So, nutrient supplements, which are enriched with natural antioxidants should be provided to overcome xenobiotic toxic stress.