Abstract
The objective of the current study was to evaluate the effects of antioxidant ergothioneine added to cryopreservation extender on DNA intergrity of Merino ram sperm. Semen samples from 5 mature Merino rams (1 and 2 years of age) were used in the study. Semen samples, which were diluted with a Tris-based extender containing ergothioneine at different doses and no antioxidant (control), were cooled to 5oC and frozen in 0.25 ml French straws. Frozen straws were then thawed at 37oC for 20 s in a water bath for evaluation. The addition of ergothioneine at doses of 1, 2 and 4 mM resulted in lower sperm with damaged DNA (5.4, 4.7 and 3.2%, respectively) than that of control (7.9%), thus reducing the DNA damage (P<0.01). Findings of this study showed that the increasing doses of ergothioneine supplementation in semen extenders, was of greater benefit to DNA integrity of frozen-thawed ram sperm.
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Schedule a callMore From: Bulletin of University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca Veterinary Medicine
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