Abstract

In this research, protease pretreated wool fabric (PWF) was treated with butane tetra carboxylic acid (BTCA), citric acid (CA), and nano TiO2 (NTO) to produce fabric with enhanced durable antimicrobial and ultraviolet (UV) protection properties. However, treatment of proteases enzymatic wool with NTO/CA/BTCA has not been informed. The NTO particles were stabilized on the enzymatic wool surface using CA/BTCA cross-linking agents. In order to create optimum UV protection (UP) based on (ΔE∗) and bacteria reduction against gram-positive and gram-negative bacteria, the appropriate models were obtained based on Design-Expert software. The roles of CA, BTCA, and NTO concentrations in UP and antimicrobial properties of the PWF were investigated using response surface methodology. Overall, the protease pretreatment of the wool fabrics made the improvement of the antimicrobial activity against Staphylococcus aureus and Escherichia coli bacteria, and also caused the remarkable enhancement of UP properties as compared with control samples. Also, reflectance spectra analyses (200–400 nm) and scanning electron microscopy were employed to confirm the NTO particles on the pretreated wool surface.

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