Abstract
DNA content in total microorganisms in plants can utilized for DNA fingerprint studies and a description of the physiological conditions of the plant. The metagenomic technique represents a novel approach in complex genomic analysis of total non-cultured microorganisms. The purpose of this study was to obtain metagenomic DNA isolated from clove plantations of communities in Ternate. The method used in this research is total DNA isolated from leaf and stem samples directly using ZymoBiomic (Zymo Research DNA Extraction) Kit, then amplified DNA based on 16s RNA with forward sequence 27F (5'-AGAGTTTGATCATGGCTCAG-3') sequence and sequence reverse ie 1492R (5'- GGTTACCTTGTTACGACTT-3 '). The results of this study show that of four samples only one sample from fresh leaves have 1400bp. The metagenomic technique of plant tissue is less effective because of many contaminants that are amplify resulting in a double peak amplicon from the first base up to the last base.
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