Abstract
Escherichia coli K12 uvrE is a mutator strain which is highly sensitive to ultraviolet (UV) radiation. In an attempt to determine the underlying molecular basis for the UV sensitivity, we have compared a mutant and an isogenic wild type strain with regard to several metabolic responses to 254-nm radiation. The introduction of single-strand breaks into intracellular DNA after irradiation is normal. However, the rate of excision of pyrimidine dimers as well as of DNA degradation and final rejoining of the strand breaks is lower in the mutant as compared to the repair proficient strain. These data suggest that the uvrE gene product may be involved in a reaction between the incision and excision steps in the excision repair process.
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