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https://doi.org/10.1007/bf00261900
Copy DOIJournal: European biophysics journal : EBJ | Publication Date: Sep 1, 1988 |
Citations: 69 |
The behavior of single chloride channels in sarcoplasmic reticulum of rabbit and trout skeletal muscle was examined by fusing isolated vesicle fractions into planar lipid bilayers. The channel exhibited a full open state with a unit conductance of 65 pS (in 100 mM Cl-) and several subconductance states with reversal potentials which were dependent on the chloride gradient across the bilayer. Open probability was 0.6-0.95 for membrane potentials ranging from -60 to +60 mV. The kinetic behaviour could be described by assuming one time constant for the fully conducting channel, and at least two time constants for the non-conducting channel. In the presence of methane sulfonate, sulfate and phosphate anions, a decrease in the unit current amplitude but not open time argued in favor of a competition between these anions and Cl- at the transport site of the channel. Chloride channel activity was not affected by variations of Ca2+ concentration in both chambers or by the presence of Mg2+. Similarly, neither millimolar ATP nor the presence of the drugs taurine (up to 10 mM), lidocaine (2-40 microM) or the calmodulin antagonist W7 (5-150 microM), modified channel behavior. Finally, pH variations between 6.8 to 8 were without effect.
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