Abstract

Different methods have been used for differentiation between Erwinia atroseptica (Van Hall) Jennison and Erwinia carotovora (Jones), Holland. This work ensured that ethyl alcohol utilization, production of reducing compound(s) from sucrose, V. P. test, gelatin liquefaction in the presence of 2% glucose, and the ability to produce typical black leg symptoms, along with serological methods, can be considered as efficient methods for the differentiation between E. atroseptica and E. carotovora isolates, supporting their classification as distinct species. However, the ability to produce tyrosinase by E. atroseptica and E. carotovora could not be proved experimentally in this work. The results also indicated that the test organisms produced polyphenol oxidase in culture media. Zur Unterscheidung von Erwinia atroseptica (Van Hall) (Jennison) and Erwinia carotovora (Jones) (Holland) wurden verschiedenste Methoden angewendet. Die vorliegende Untersuchung bestätigte, daß die Verwendung von Äthylalkohol, die Erzeugung von reduzierenden Verbindungen aus Sucrose, der V. P.-Test, Gelatineverflüssigung bei Anwesenheit von 2% Glukose und die Fähigkeit zur Erzeugung der typischen “black leg” Symptome, zusammen mit serologischen Methoden, als eine wirksame Methode zur Unterscheidung von Erwinia atroseptica und Erwinia carotovora betrachtet werden kann und deren Klassifizierung als unterschiedliche Spezies bestätigt. Bei diesen Untersuchungen konnte jedoch experimentell nicht erwiesen werden, daß E. atroseptica und E. carotovora die Fähigkeit besitzen, Tyrosinase zu erzeugen. Die Ergebnisse zeigen such, daß die Testorganismen in den Kulturmedien Polyphenoloxydase erzeugten.

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