Abstract
Shoot tips 0.5mm long were taken from actively growing almond (Prunus amygdalus Batsch cv. hardy) shoots. These were cultured on the modified 1/2 MS medium (UEMATSU and AKIHAMA 1987) supplemented with 6-benzyladenin (6-BA) or N-(2-chloro-4-pyridyl)-N-phenylurea (4PU, Kyowahakko Kogyo K. K.). About 70 to 80% of shoots survived on the paper-wick liquid medium containing 2.0 or 4.0 mg/l 4PU. Growing shoots were transferred to the solid multiplying medium containing 2g/l gerlite and 0.5mg/l 6-BA. These shoots were subcultured on the same fresh medium at forty days intervals. After nine months of culture, the multiplication rate was 6.6-fold per L0rty days. The longest shoot reached to 3.8cm. Within a month after transferring to the rooting medium containing 0.5mg/l naphthalene aceticacid (NAA), root induction occurred.
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