Propagation and in vitro studies of previously non-cultivable enteral adenoviruses in 293 cells.

Abstract

Enteral adenovirus (EAd) isolates which characteristically fail to propagate in several human cell lines grow readily in 293 cells, an adenovirus type 5 transformed line. EAd infection of 293 cells is associated with high levels of 31S DNA and late viral protein synthesis, the appearance of a progressive adenovirus-like cytopathic effect, and the efficient assembly of progeny virions. The DNA of an EAd strain grown in 293 cells possesses restriction endonuclease profiles similar to those reported for EADs recovered directly from stools. In their ability to replicate efficiently in 293 cells but not in other human cell lines. EAds appear to behave like adenovirus host range mutants.