Abstract
TheAgrobacteriumT-cytgene was transferred into lettuce,Latuca sativa‘Saladin’ using a genotype-independent transformation procedure employing a supervirulentAgrobacterium tumefaciensstrain carrying the binary vector pMOG23. Kanamycin-resistant shoots were initiated from inoculated explants only when sites were deleted within the T-cytpromoter. In culture, kanamycin-resistant shoots exhibited several phenotypes, including gall production, reduced internode length (dwarfism) and vitrification together with differences in rooting. Rooted, neomycin phosphotransferase (NPTII)-positive plants recovered from their abnormal phenotype and self pollinated to produce viable seed, following transfer to the glasshouse. Kanamycin-resistant lettuce plants exhibited increased cytokinin and chlorophyll contents compared to non-transformed plants, physiological features which could benefit long-term storage of this leafy vegetable.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
