Promising photoluminescence optical approach for triiodothyronine hormone determination based on smart copper metal–organic framework nanoparticles

Abstract

A copper metal–organic framework nanoparticles (Cu‐MOF‐NPs) synthesized via simple technique. The prepared Cu‐MOF‐NPs nanoparticles were further characterized using 1H‐NMR, FE‐SEM/EDX and thermal study (DSC/TGA). The FE‐SEM/EDX, thermal analysis, and NMR spectrum data with the other analysis support the nano‐Cu‐MOF structure and the monomeric unit (n[Cu (AIP)2(APY)(H2O)2].4H2O) of Cu‐MOF‐NPs. The photoluminescence (PL) studies of triiodothyronine hormone (T3) based on the prepared Cu‐MOF‐NPs investigated. The results revealed that the Cu‐MOF‐NPs might be used as a biosensor in the determination of triiodothyronine hormone (T3) in biological fluids through a significant quenching of the photoluminescence intensity of Cu‐MOF‐NPs at excitation wavelength 492 nm. The calibration plot achieved over the concentration range 0.0–200.0 ng/dL T3 hormone with a correlation coefficient 0.996 and limit of detection (LOD) and quantification (LOQ) 0.198 and 0.60 ng/dL, respectively. The PL spectra are indicating that Cu‐MOF‐NPs has highly selective sensing properties for T3 hormone without interfering with other human many hormones types. This approach considered a promising analytical tool for early diagnosis of the cases of thyroid disease. The mechanism of quenching between the Cu‐MOF‐NPs, and T3 hormone studied. The mechanism was a dynamic type and obtained due to the energy transfer mechanism.