Prokaryotic Expression of SGEG1 Protein from Artemia Diapause Cysts

Abstract

Artemia is a small marine animal survived in extreme environments. Its diapause cysts can maintain long-term vitality in dry and anoxic environment and develop into larvae under suitable condition. Artemia passed through the extreme environment by its dormancy state. The shell gland-specifically expresses gene (SGEG) is specifically expressed in secretory cells of shell gland of oviparous Artemias ovisac and SGEG peptide is an important componenProkaryotic Expression of SGEG1 Protein from Artemia Diapause Cystst of diapause cysts shell protein. In this study, the SGEG1 gene sequence of deduced mature peptide was cloned into the prokaryotic expression vector pET-28a (+) with 6×His tag. A recombinant plasmid containing pET-28a (+) -SGEG1 was successfully constructed and transformed into E. coli BL21(DE3). The fusion expression of recombinant SGEG1 (rSGEG1) protein was induced. Meanwhile, the induced IPTG concentration, induction time of rSGEG1 and the method of protein electrophoresis were optimized. The recombinant SGEG1 peptide with a molecular weight of about 10 K Da was highly expressed,and the fusion protein mainly existed in the form of inclusion body, which supported a foundation for the preparation of SGEG1 protein in vitro.