Abstract
Duck circovirus (DuCV), as a causative agent of long-term immunosuppressive disease, has caused heavy damage to waterfowl breeding worldwide. In this study, the full-length Cap (capsid) gene of DuCV was expressed in Escherichia coli (E. coli) for the first time by optimizing the codons in its nuclear localization signal (NLS) regions. The recombinant protein was expressed as inclusion bodies, and the quantification of purified Cap protein could reach 0.29mgmL−1. Moreover, an indirect ELISA method (Cap-ELISA) was established based on the recombinant Cap protein. The results of the optimization for Cap-ELISA revealed that the optimum concentration of the coating antigen and serum dilution ratio were 19.5ng per well and 1:1280, respectively. The cut-off value of the Cap-ELISA for positive sample detection was 0.145, the sensitivity of that reached 1:25600, and it was specific for the detection of DuCV anti-sera. In comparative experiments using 56 clinically suspected DuCV-infection samples, the Cap-ELISA showed a 94.64% coincidence rate with the PCR test. These results indicated that codon optimization is a reasonable strategy to obtain an intact Cap protein, and this Cap-ELISA is suitable for extensive applications in DuCV serological diagnosis.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
