Abstract
Molecular biology techniques have been applied to study the diversity and biomass of metabolically active prokaryotic cells in an oil-contaminated oligotrophic peat soil at different levels of mineral nutrition. The share of metabolically active components in the peat samples is only about 10% of the entire prokaryotic community. The application of mineral fertilizer (N40P50K50) against the background of half-dose liming has led to a more than twofold increase in the biomass of bacterial and archaeal cells, an increase in the number of functional genes (bss and nifH) copies, and a significant decrease in the content of oil products in the peat of experimental variants. The application of mineral fertilizer against the background liming of oil-to contaminated soil induces changes in the phylogenetic structure and partial restoration of the metabolically active prokaryotic complex.
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