Progressivity of TNF-α production and miR-29b-3p expression during hypercholesterolemia

Abstract

Hypercholesterolemia triggers atherosclerosis, characterized by releasing TNF-α and miR-29-3p that may be applicable for biomarker or therapeutic targets. This study was aimed to analyze the correlation between TNF-α production and miR-29b-3p expression during hypercholesterolemia progression. Total 12-male-Sprague Dawley rats weighted of 170-180 gr were randomly divided into normal control group and hypercholesterolemia (HC) group, equally. The Sprague Dawley (SD) rats were given only standard feed for seven weeks, combined with placebo for normal group and 2 ml of cholesterol/200 grBM/day, orally for hypercholesterolemia group. The miR-29b-3p expression analysis was performed by qRT-PCR and TNF-α using ELISA, by followed manufacture procedures. The miR-29b-3, then analyzed using Kruskal-Wallis analysis, whereas TNF-α was analysed using t-test. During, initial week, TNF-α was produced 5.19±0.20 pg/ml in the normal group and significantly different with HC group there was 4.43±0.32 pg/ml, then it significantly increases up to 6.12±0.21 pg/ml in the normal group and 14.10±0.33 in the HC group. Increased of miR-29b-3p expression was occurred in the normal group by from 1.33±0.24-fold change in 1st week to 2.55±0.08 fold change in 8th week, then 1.66x104±1.06x104 to 1.94x104±1.19x104 fold change in HC group.