Abstract
Pretreatment is the key step to overcome the recalcitrance of lignocellulosic biomass making sugars available for subsequent enzymatic hydrolysis and microbial fermentation. During the process of pretreatment and enzymatic hydrolysis as well as fermentation, various toxic compounds may be generated with strong inhibition on cell growth and the metabolic capacity of fermenting strains. Zymomonas mobilis is a natural ethanologenic bacterium with many desirable industrial characteristics, but it can also be severely affected by lignocellulosic hydrolysate inhibitors. In this review, analytical methods to identify and quantify potential inhibitory compounds generated during lignocellulose pretreatment and enzymatic hydrolysis were discussed. The effect of hydrolysate inhibitors on Z. mobilis was also summarized as well as corresponding approaches especially the high-throughput ones for the evaluation. Then the strategies to enhance inhibitor tolerance of Z. mobilis were presented, which include both forward and reverse genetics approaches such as classical and novel mutagenesis approaches, adaptive laboratory evolution, as well as genetic and metabolic engineering. Moreover, this review provided perspectives and guidelines for future developments of robust strains for efficient bioethanol or biochemical production from lignocellulosic materials.
Highlights
Due to the exhaustibility of fossil fuels and its indiscriminate use, there is an urgent need for the development of sustainable and affordable alternatives
Zymomonas mobilis has being developed as an effective model for biofuel and biochemical production from lignocellulosic materials
Microbial fermentation is still commonly affected by the presence of toxic inhibitors in the hydrolysate, which severely impeded its industrial application (Franden et al 2013; Yi et al 2015)
Summary
Due to the exhaustibility of fossil fuels and its indiscriminate use, there is an urgent need for the development of sustainable and affordable alternatives. It is critical to examine the toxic effect of hydrolysate compounds on cell growth and fermentation performance to help elucidate inhibitor tolerance mechanisms and to allow the development of robust industrial strains for economic biochemical production.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.