Abstract
The purpose of this study was to develop a method to highlight immune cells and cell clusters in histology tissue stained with single or multiple biomarkers. B6 mice were implanted (rear flank) with YUMM/YUMMER1.7 melanoma cells and treated with or without immunotherapy (PD-1, CTLA-4). Skin, lymph nodes, and spleens were formalin-fixed paraffin-embedded and stained by
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