Abstract
Research about chromatographic profile of antioxidant and antibacterial compounds in n-hexane fraction of Libo leaves (Ficus variegata Blume.) has been done in order to know the form of chromatographic profile of antioxidant and antibacterial compounds in n-hexane fraction of Libo leaves. Extraction was done by maceration using methanol. Isolation was done by 2 methods, vacuum liquid chromatography (VLC) then followed by conventional column chromatography. Eluent used are n-hexane-ethyl acetate and methanol-chloroform. The results based on conventional column chromatography are as many as 10 fractions. The fractions are being conducted then by a qualitative testing of the antioxidant activity using DPPH method and antibacterial activity test using bioautography TLC method. The results formed as a chromatographic profile of fraction that contain antioxidant and antibacterial activity, which are as many as 5 fractions (NHB1, NHB3, NHB4, NHC1, NHC3) active as an antioxidant and 4 fractions (NHB1, NHC1, NHC2, NHC3) which are active as an antibacterial. The secondary metabolite contents of the fractions are alkaloid, flavonoid and steroid/terpenoid.
Highlights
Penelitian mengenai profil kromatografi senyawa antioksidan dan antibakteri dalam fraksi nheksana dari daun Libo (Ficus variegata Blume.) telah dilakukan dengan tujuan untuk mengetahui profil kromatografi senyawa antioksidan dan antibakteri dalam fraksi n-heksana dari daun Libo
in order to know the form of chromatographic profile
Extraction was done by maceration using methanol
Summary
Indonesia telah banyak yang diketahui memiliki khasiat pengobatan. Beberapa contoh potensi senyawa aktif yaitu sebagai antioksidan dan antibakteri. Antioksidan merupakan senyawa yang berguna untuk menstabilkan radikal bebas [1]. Radikal bebas ialah suatu molekul yang memiliki jumlah elektron ganjil atau elektron tak berpasangan pada lingkaran luarnya. Libo (Ficus variegata Blume.) merupakan tumbuhan liar yang belum banyak dikenal dan pengetahuan akan manfaatnya juga masih sangat minim. Rijai [4] menyebutkan beberapa potensi buah Libo yaitu sebagai sumber bahan larvasida untuk Aedes aegepti, sitotoksik (antikanker), antioksidan dan antibakteri. Sedangkan pengetahuan mengenai potensi daun Libo masih sangat kurang. Oleh karena itu dilakukan penelitian mengenai potensi senyawa aktif yang terdapat pada daun Libo, khususnya potensi senyawa aktif antioksidan dan antibakteri pada fraksi n-heksana. Daun Libo juga memiliki potensi aktivitas yang tak jauh berbeda
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