Abstract

An abundant amount of agricultural/horticultural waste were generated from agricultural and industrial processing. However, such wastes usually have a composition rich in sugars, minerals and proteins, and therefore, they should not be considered "wastes" but raw materials for other industrial processes. The purpose of the present work was to optimize the xylanase production by Myceliopthora thermophila SH1 (Ascomycota: Chaetomiaceae) an alkalothermophilic strain isolated from hot spring of Himachal Pradesh under solid state fermentation using different agricultural wastes/horticultural waste as a substrate by enrichment with basal salt medium at temperature 45 oC after incubating it for 7 days. The highest level of enzymes was produced using rice bran, orange pulp, sugarcane bagasse, wheat straw, wheat bran, apple pomace as a substrate. The secreted extracellular enzyme presented a property that matches the requirement in industrial environment.

Highlights

  • Thermophilic microorganisms have gained a great deal of attention in the last two decades (Beg et al, 2000)

  • The total recovered activity of xylanase in orange pulp was higher as compared to other substrates

  • The yield was 161.20 U/gds in orange pulp as a substrate after that the sugarcane with 131.40 U/gds of xylanase was recommended as an effective substrate for xylanase production under solid state fermentation after 5 days of incubation

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Summary

Introduction

Thermophilic microorganisms have gained a great deal of attention in the last two decades (Beg et al, 2000) Enzymes produced from these microorganisms are of special interest since these are not usually denatured by high temperature and are even active at elevated temperature. Interest in these microorganisms has increased worldwide owing to their potential commercial application namely production of bioactive compounds, including antibiotics and enzymes (Malherbe and Cloete, 2002; Lynd et al, 2005). Sci. http://revista.rebibio.net because of their wide application in many fields These enzymes fetch extraordinary high prices in the market and main factor responsible for that is the use of expensive substrates like pure cellulose and xylan for production of enzymes

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