Abstract
The asymmetric microbial reduction of phenylglyoxylic acid (PGA) to ( R)-mandelic acid (( R)-MA) with immobilized Saccharomyces cerevisiae cells on globular chitosan was studied. The immobilization conditions and characterization of the immobilized cells were carried out. Chitosan–acetic acid solution was injected into a mixture of 20% NaOH and 30% CH 3OH aqueous solution to obtain globular chitosan, and then the globular chitosan was treated with 1% solution of glutaraldehyde to immobilize yeast cells, which were used to synthesize ( R)-MA. The optimum conditions were identified as the substrate concentration of 10 mmol L −1, pH of 6.5 and reaction temperature of 30 °C with the yield of 62% for ( R)-MA and the enantiomeric excess (e.e.) of 98% for ( R)-MA. The immobilized cells showed good operation and storage stability.
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