Abstract
Raoultella ornithinolytica YNKP001 and Leclercia adecarboxylata P10164, which harbor conjugative plasmids pYNKP001-NDM and pP10164-NDM, respectively, were isolated from two different Chinese patients, and their complete nucleotide sequences were determined. Production of NDM-1 enzyme by these plasmids accounts for the carbapenem resistance of these two strains. This is the first report of blaNDM in L. adecarboxylata and third report of this gene in R. ornithinolytica. pYNKP001-NDM is very similar to the IncN2 NDM-1-encoding plasmids pTR3, pNDM-ECS01, and p271A, whereas pP10164-NDM is similar to the IncFIIY blaNDM-1-carrying plasmid pKOX_NDM1. The blaNDM-1 genes of pYNKP001-NDM and pP10164-NDM are embedded in Tn125-like elements, which represent two distinct truncated versions of the NDM-1-encoding Tn125 prototype observed in pNDM-BJ01. Flanking of these two Tn125-like elements by miniature inverted repeat element (MITE) or its remnant indicates that MITE facilitates transposition and mobilization of blaNDM-1 gene contexts.
Highlights
Raoultella ornithinolytica is widely found in aquatic environments, insects and fishes
Determination of Minimum Inhibitory Concentration (MIC) The MIC values of the indicated bacterial strains were tested by VITEK 2 according to the manufacturer’s instructions, and antimicrobial susceptibility was assessed by the Clinical and Laboratory Standards Institute (CLSI) standards
Characterization of R. ornithinolytica YNKP001 and L. adecarboxylata P10164 Raoultella ornithinolytica YNKP001 was recovered in November 2010 from the blood specimens of a 4-year-old child with acute encephalitis, bronchitis and tympanitis from a hospital in Kunming City in China
Summary
Raoultella ornithinolytica is widely found in aquatic environments, insects and fishes. R. ornithinolytica is able to convert histidine to histamine (scombroid toxin) and is known to cause fish poisoning. Infections by R. ornithinolytica are exceedingly rare in humans and have been reported as bloodstream, urinary tract and soft tissue infections in adults and as fatal neonatal infections. Most adult cases are linked with underlying diseases, especially malignancies (Morais et al, 2009; Mau and Ross, 2010; Solak et al, 2011; Hadano et al, 2012; Haruki et al, 2014; Chun et al, 2015). Raoultella ornithinolytica produces at least two different chromosomally encoded class A β-lactamases. R. ornithinolytica is resistant to ampicillin but commonly remains susceptible to cefotaxime and imipenem (Walckenaer et al, 2004). Carbapenem-resistant blaNDM-1 in R. ornithinolytica and L. adecarboxylata
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
