Abstract
The leaf disks of Nicotiana plumbaginifolia were irradiated by an 14N heavy-ion beam and then were cultured in shoot-inducing medium in order to produce mutants for elucidating the functions of the cell wall in plant morphogenesis. Non-organogenic and loosely attached callus, which had the same morphological features as non-embryogenic callus of carrot was formed on 11.8% of the haploid leaf disks irradiated by 14N heavy-ion beam (5 Gy) at high frequency. The cells stimulated by the heavy-ion beam loosely attached to each other resulting in a random morphology of cell clusters, whereas the non-irradiated cells formed a tight callus with multiple shoots.
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