Production of Monoclonal Antibodies against Common Epitopes of Salmonella by Oral Immunization of Laboratory Animals

Abstract

Materials and Methods Twelve mice were divided into two groups and immunized orally with S. suberu (3.10: g, m) and S. strasburg from the OD2 group (O:9.46). The Kohler and Milstein method yielded IgA and IgM class IgA secreting monoclonal antibodies. They were characterized by the methods of slide-agglutination, haemagglutination, ELISA, immunoblotting. Results Four anti-flagelar MAbs of class IgA and one anti-O-class IgM class were obtained. All of the IgA class reacted with the Hg epitope of flags obtained from Salmonella strains and S. enteritidis flagellin. In IgMMAbs, only O-antigens isolated from Salmonella strains belonging to the OD2 group were present. Conclusion Through this method of oral immunization, besides highly specific IgA MAbs, we also received a clone of hybridomas expressing specific IgM MAbs which by ELISA and SAT show excellent opportunity to participate in diagnostic reactions against salmonellae with a common group epitope OD2.