Production of Interspecific Germline Chimera between Quail and Chicken Utilizing the Cells from Central Disk of Blastoderm and Germinal Crescent Region

Abstract

Production of interspecific germline chimera between quail and chicken was carried out using the cells from the central disk (CD) of the area pellucida in quail blastoderm (stage X) and the germinal crescent region (GCR) of quail embryo (stage 7-8). The cells of CD and GCR were dispersed and injected into the subgerminal cavity of chicken blastoderm (stage X). Injected eggs were incubated for 7 days or to hatching. To detect quail genomic DNA, the polymerase chain reaction was used. In day-7 embryos, quail DNA was detected in 4 gonads and 6 brains from 55 survived embryos received quail CD cells, and 9 gonads and 8 brains from 68 survived embryos received quail GCR cells. Quail DNA was also detected from eggs or semen of adult female or male chicken hatched from eggs received quail CD or GCR cells. CD and GCR cells as the donors showed the possibility to produce the interspecific germline chimera but the necessary improvement and the immunoreactive problem were suggested.