Abstract
Sponge metagenomes are accessible genetic sources containing genes and gene clusters responsible for the biosynthesis of sponge-derived bioactive natural products. In this study, we obtained the clone pDC112, producing turbomycin A and 2,2-di(3-indolyl)-3-indolone, based on the functional screening of the metagenome library derived from the marine sponge Discodermia calyx. The subcloning experiment identified ORF 25, which is homologous to inosine 5'-monophosphate dehydrogenase and required for the production of 2,2-di(3-indolyl)-3-indolone in Escherichia coli.
Highlights
Marine sponges are prolific sources of bioactive molecules as well as highly complex consortia, including significantly large populations of symbiotic bacteria.[1]
We obtained the clone pDC112, producing turbomycin A and 2,2-di(3-indolyl)-3-indolone, based on the functional screening of the metagenome library derived from the marine sponge Discodermia calyx
We obtained the red-pigmented clone pDC112, producing antibacterial substances, by the functional screening of the metagenome library generated from the marine sponge D. calyx
Summary
Marine sponges are prolific sources of bioactive molecules as well as highly complex consortia, including significantly large populations of symbiotic bacteria.[1]. We recently reported the biosynthetic gene cluster of a cytotoxic compound, calyculin A, which is composed of NRPS-PKS hybrid genes encoded by an uncultured sponge symbiont, Candidatus ‘Entotheonella sp.’.5
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