Abstract
Anther culture was used to produce haploid plants from Aegilops variabilis ssp. euvariabilis × Secale cereale (inbred line) amphiploids (2n=42) and their parental forms. The anthers were cultured on modified induction media - C 1 7 and PII. Androgenetic embryos were obtained only from amphiploid plants and the frequency depended on genotypes. On C 1 7 medium 20.0 - 65.2 and on PII medium 1.4 - 15.7 androgenetic embryos /100 anthers were produced. The embryos were transferred to a regeneration medium 190-2 and 0.1 - 13.4 of green plants /100 androgenetic embryos were obtained. The haploid (n=21) chromosome number was found in all plants. Doubled haploids were produced by haploid colchicine treatment.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
