Abstract
Background The envelope protein (E protein) of dengue virus is responsible for binding the virus to the host cell. This protein is considered an important immunogen for neutralization of the virus, the only able to induce the production of neutralizing antibodies [1]. The aim of this study was to use the Cowpea Mosaic Virus (CPMV) as a vector to express, on cowpea plants, Vigna unguiculata L. (Walp) the gene fragment that encodes for domain III of the E protein of dengue 2 virus.
Highlights
The envelope protein (E protein) of dengue virus is responsible for binding the virus to the host cell
The cloning of inserts in non commercial plasmid (NCP) specific sites containing the RNA-2 of Cowpea Mosaic Virus (CPMV), its subsequent introduction into competent cells of Escherichia coli (DH10B) and the purification of these plasmids from the transformed cells was made through conventional molecular biology techniques
The chimerical virus was inoculated on cowpea plants, and the symptomatic leaves were processed for further purification of the recombinant protein
Summary
The envelope protein (E protein) of dengue virus is responsible for binding the virus to the host cell.
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
