Abstract
Prebiotics is indigestible foods that can not be digested but can stimulate the growth and activity of bacteria in the digestive tract effecting human health. Alginate oligosaccharides (AOS) can be used as a source of prebiotic. That compounds can be produced enzymatically by cutting long chain alginates using alginate lyase. The aim of this study was to produce alginate lyase enzyme then producing Alginate oligosaccharides (AOS) as a prebiotic ingredients. The alginate lyase enzyme can be produced from Bacillus megaterium bacteria using a discontinuous fermentor. The enzyme was optimum temperature of 45°C and an optimum pH of 7.0. Alginate oligosaccharides production was performed with the addition of different enzyme concentrations 25, 50, 75, and 100 U. The result of the addition of enzyme (25, 50,75 U) showed that the value of polymerization degrees (DP) were between 4-5. However, the addition of enzyme (100 U) was in the range of DP 3-4. Bacterial probiotic growth test results of Bifidobacteria and Lactobacillus showed that 1% added AOS media were able to increase the growth of probiotic bacteria compared to the<br />media without addition of AOS. The addition Alginate lyase activity of 50 U in AOS production is the best treatment of both probiotic bacteria. <br /><br />
Highlights
Prebiotik merupakan serat larut air atau bahan makanan yang tidak dapat dicerna oleh enzim pencernaan tetapi dapat menstimulasi pertumbuhan dan aktivitas bakteri di dalam sistem pencernaan
The alginate lyase enzyme was produced by Bacillus megaterium bacteria using a discontinuous fermentor
Alginate oligosaccharides production was performed with the addition of different enzyme activities (25, 50, 75, and 100 U)
Summary
Tepung alginat dari Sargasum crassifolium, NaCl (Merck, Darmstadt, Germany), KH2PO4 (Merck, Darmstadt, Germany), Amonium Sulfat (Merck, Darmstadt, Germany), MgSO4.7H2O (Merck, Darmstadt, Germany), 1-butanol (Merck, Darmstadt, Germany), asam format (Merck, Darmstadt, Germany), akuades, etanol (Merck, Darmstadt, Germany), pereaksi Bradford (Sigma-Aldrich, Missouri, USA), bufer fosfat pH 7 Alat yang digunakan dalam penelitian yaitu Autoclave Hirayama HVA 110, Beaker glass, Centrifuse LMC-4200R, Erlenmeyer, Fermentor Masterflex 7518, Hot plate Cimarex, Laminar Flow Esco OptimairTMVertical, micropipette, petri dish, spektrofotometer UVVis InScienpro Lab Equitment 325-1.000nm, Thin Layer Chormatography (TLC) dan pH meter Jenway 3510
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