Abstract
Bacillus circulans AB 16 was able to produce 50 IU/ml of xylanase, with negligible cellulase activity when grown on untreated wheat straw. The pH optimum of the crude enzyme was 6–7 with a temperature optimum of 80 ∘C. The enzyme showed high pH and thermal stability retaining 100% activity at 60 ∘C, pH 8 and 9 after 2.5 h of incubation. The residual activity at 70 ∘C after 2.5 h was 62% and 45% at pH 8 and 9, respectively. At 75 ∘C only 22.2% activity remained at pH 8 after 1 h incubation. Since Kraft pulp is alkaline this enzyme could be used for prebleaching of pulp at temperatures up to 70 ∘C without pH adjustment.
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