Production of a new mucilage compound in Lepidium sativum callus by optimizing in vitro growth conditions

Abstract

The mucilage in Lepidium sativum L. is considered a biologically active compound with diverse medicinal properties. Different explants (hypocotyls and leaf) were transferred to Murashige and Skoog (MS) medium supplemented with twelve different plant growth regulator combinations under two different incubations (light and dark). The best mucilage production from callus (36.76% g g−1 dry weight) was obtained in the MS medium supplemented with 1 mg L−1 of 2, 4-D and 2 mg L−1 of BAP under the light condition. The mucilage produced by callus culture was nearly three times more than the mucilage yield of the seeds. The glucose, arabinose + mannose and galactose were 43.4 (mg g−1 DW), 195.3 (mg g−1 DW) and 86.2 (mg g−1 DW) in the mucilage originated from seed, callus leaf and callus hypocotyl, respectively. The present study proposes an efficient method for producing large scales of mucilage with a favorable sugar aimed at food or pharmaceutical industries.