Probiotics on Intestinal Flora Disturbance and Bacterial Translocation in Mice with Inflammatory Bowel Disease

Abstract

Inflammatory bowel disease is becoming more and more common in western countries and less common in China. However, with the change of dietary structure, the incidence of inflammatory bowel disease is also increasing in China. It will cause abdominal pain, diarrhea and mucus, pus and blood, which are repeatedly delayed and difficult to alleviate. To explore the effectiveness of probiotics in the treatment of inflammatory bowel disease, Lactobacillus plantarum in probiotics and fifteen 8 w old female mice were selected for the experiment. We divided the mice into three groups: blank control group, interleukin-10 group and interleukin-10+Lactobacillus plantarum group. The interleukin-10+Lactobacillus plantarum group was gavaged with 0.5 ml of Lactobacillus plantarum bacterial solution (1.0×109 CFU/ml) every d. The blank control group and interleukin-10 group were gavaged with 0.5 ml of Ringer buffer for 4 consecutive w and compared the changes caused by Lactobacillus plantarum on Bifidobacterium, Lactobacillus, Clostridium perfringens and Enterobacter in the intestinal flora of mice and the changes caused by Lactobacillus plantarum on the intestinal flora effect of bacterial translocation in membranous lymph nodes and spleen. We found that the content of Enterobacter in the blank control group was the lowest at all three time points, with an average of 6. The content of Enterobacter in the interleukin-10+Lactobacillus plantarum group and the interleukin-10 group was 6.5 and 6.6 respectively, at the beginning of 0 w and 6.3 at the beginning of 2 w but after the 4 w, the content of Enterobacter in the two groups became 6.4 and 6.7, the difference increasedy.