Abstract
Abstract The use of in situ hybridization for the detection of messenger RNA (mRNA) in tissue sections and other types of tissue or cell preparations is widely accepted as being a powerful technique for investigating gene expression at the cellular level. The high specificity and sensitivity of the bond formed between the nucleic acid probe and its target in the tissue section allows the detection of low abundance mRNAs, although there is a detection limit for the technique. In this chapter, the use of complementary RNA (cRNA) probes will be discussed (for a discussion of other types of probes, see Chapters 1-3).
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