Primer Design and Sequence Information for Cytochrome b Gene in the Chinese Painted Quail (Excalfactoria chinensis)

Abstract

In accordance with the chicken and quail mitochondrial DNA sequences, three pairs of primers encompassing light and heavy strand (L14832, L15269, L15500, H15924, H15497, and H16207) with high specificity were designed, synthesized, and used for amplification and sequencing of cytochrome b (Cyt b) gene. The PCR products were sequenced by the direct-sequencing method. The Chinese painted quail cytochrome b gene consisted of 1, 143bp nucleotides (base composition: 327 A; 399 C; 281 T and 136 G), encoded for a 380 amino acid sequence, and showed 89.0%, 86.4%, and 86.2%, homology with nucleotide sequences of Japanese quail, silky fowl, and White Leghorn chicken, respectively. The homology of the amino acid sequence of the cytochrome b gene of Chinese painted quail showed 98.2%, 95.3%, and 95.3% with those of Japanese quail, silky fowl, and White Leghorn chicken, respectively. The sequences reported in this paper have been deposited in the GenBank database (Accession No. AF109217).