Abstract
SummaryBy employing conventional husbandry techniques and filter-capped cages, a breeding colony of mice from Caesarian-derived stock was maintained free from many infectious agents common to laboratory mice through seven generations. The system described prevented infections with pathogenic bacteria, viruses, parasites, and PPLO. Mice housed under these conditions remained healthy and fecund while being free of the morphological and physiological consequences of germfree environment. In addition, the expense and restrictions of conventional barrier-type operations were minimized.
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