Abstract
The decision to enter pupal diapause can be reversed in the flesh fly Sarcophaga crassipalpis by treatment with a juvenile hormone analogue, ecdysterone, or cholera toxin. A topical application of juvenile hormone analogue (10μg) is effective in preventing diapause if applied to newly ecdysed 3rd instar larvae. An ecdysterone injection (1μg) into post-fed 3rd instar larvae can avert diapause, and even lower dosages (0.01μg) are effective if injected into young pupae immediately before the onset of diapause. Cholera toxin, a stimulant of adenylate cyclase, can prevent diapause if 1μg is injected into larvae 24 hrs. prior to pupariation. This is the first report of an effect of cholera toxin on insects. A 2-day exposure to 33°C was the only physical manipulation shown to be capable of reversing the previous commitment to enter pupal diapause.
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