Abstract
Pseudomonas aeruginosa is an opportunistic pathogen which produces several diseases and considered all over the world as one of the most dangerous diseases which affect animals. A total of thirteen isolates were identified as P. aeruginosa with percentage of 6.5%, 7.3% from diseased and dead chicken and 4% from one day old chicks. Polymerase chain reaction (PCR) for detection of oprL gene of P. aeruginosa was applied and showed positive amplification of 504 pb fragments. The isolates were found to be resistant for Neomycin, Nalidixic acid and Lincomycin followed by Trimethoprim-sulfamethoxazole, Chloramphenicol then Tetracycline and Doxycyclin but appeared sensitive to Levofloxacin with percentage of 100%, 92.3% to Norfloxacin, 84.6% to Ciprofloxacin, 77% to both Colistin sulphate and Gentamicin but 61.5% to streptomycin. In experimental infection for all isolated strains via yolk sac in 7 days SPF eggs and via subcutaneous injection in 3 days old chicks which expressed the virulence of these strains by its high mortality rate which reached 100% within 24-72 hrs.
Highlights
Pseudomonas aeruginosa is one of the species belonging to genus Pseudomonads which included in Pseudomonaceae family
The mortality rate by P. aeruginosa is higher than other gram negative pathogens because it has ability to produce several extracellular products that after colonization can cause extensive tissue damage and invaded blood and Corresponding author: Dr HEBA BADR E-mail address: drheba_badr@yahoo.com Present address: Reference Laboratory for Veterinary Quality Control on Poultry Production, Animal Health Research Institute, Ministry of Agriculture, Nadi El-Seid Street, Dokki P.O
In our study the conventional Polymerase chain reaction (PCR) for oprL gene which had done from the enriched isolated strains revealed that all the isolates were confirmed by the polymerase chain reaction (Fig. 2)
Summary
Pseudomonas aeruginosa is one of the species belonging to genus Pseudomonads which included in Pseudomonaceae family. It is gram negative bacteria, moving with polar flagella and produces various fluorescent pigments (Tamer et al, 1989). Among these extracellular products are alkaline protease, elastase, haemolysins, phospholipase "C". Virulence of this organism appears multifactorial, its cellular products and extracellular products ensure its ability to infect most hosts (Herbert, 2003 and Ali et al, 2009). P. aeruginosa is resistant to many widely used antibiotics which resulted from an impermeable outer membrane and the production of extracellular polysaccharides (Quinn, 1992). Vol 62 No 151 October 2016, 119-126 the phenotypic and genotypic character of isolates detection of its pathogenicity in vivo in chicken embryos and one day old chicks
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