Abstract
Background: Giant shrimps (Penaeus monodon), an important native of the aquatic food chain and abundant crustaceans in Malokun sea, Ilaje, Ondo state, serves as significant seafood recipe. This study investigated the incidence and antibiotic susceptibility signature of Vibrio species isolated from crayfish sample from Malokun sea, Ilaje, Ondo State so as to evaluate its potential as reservoir of Vibrio species in this environment. Method: A total of 30 fresh giant shrimp samples were obtained from Malokun sea and densities of Vibrio spp. were determined using MPN-PCR method. Shrimp samples were macerated in sterile blender and aliquots were processed using thiosulfate-citrate-bile salts-sucrose agar. The presumptive isolates were further confirmed as Vibrio spp. using PCR assay and then subjected to antibiotic susceptibility testing against a panel of 8 commonly used antibiotics using Kirby Bauer method. Results: Twenty (20) presumptive Vibrio isolates were randomly selected for molecular confirmation and 12 isolates were confirmed genotypically as Vibrio species. High antibiotic resistance was observed against penicillin (100%), ceftazidime (91.66%), cefepime (91.66%) doripenem while susceptibility to norfloxacin (75%), amikacin (100%), kanamycin (83.33%), streptomycin (66.66%) was likewise observed respectively. The multiple antibiotic resistance index (MARI) among Vibrio spp. ranged between MARindex 0.25-0.625 with highest MARI of 0.625 observed in 33.33% of Vibrio species showing resistance to five antibiotics. Conclusion: This study revealed high prevalence of Vibrio species and multiple antibiotics resistance among examined marine shrimps in Malokun sea, Ilaje, Ondo state. as well as raised resistance against samples. We therefore concluded that the shrimps might pose health risk if undercooked contaminated shrimps were consumed and are potential reservoir of Vibrio species which could cause Vibrio associated infections in sea animals in this marine environment. Keywords: Vibrio spp., Shrimps, Penaeus Monodon, antibiotic resistance, Nigeria
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