Prevalence and antibiogram of pseudomonas aeruginosa and acinetobacter baumannii in the clinical samples from tertiary care hospital

Abstract

Introduction: The present study was undertaken to determine the prevalence of non-fermenting gram negative bacilli and antibiogram of Pseudomonas aeruginosa and Acinetobacter baumannii. Materials and Methods: Conventional bacteriological methods were used for identification non fermenting gram negative bacilli and susceptibility testing was performed with the help of the Kirby-Bauer disc diffusion method. Results: A total 195 non fermenting gram negative bacilli were isolated during the study period. Pseudomonas aeruginosa and Acinetobacter baumannii isolates represent 89.23% of all isolated bacteria. Therefore, antibiotic resistance rates were performed specifically against these two bacteria. P. aerurginosa showed good sensitivity to imipenem(94.29%) followed by amikacin(86.67%) and piperacillin/tazobactum(68.57%). A. baumannii showed good sensitivity to imipenem(82.61%) followed by amikacin(76.81%). Conclusion: P. aeruginosa and A. baumannii were the most common NFGNB isolated in our study. Both showed good susceptibility towards imipenem and amikacin. Alarming resistance rate was found towards commonly used antibiotics. Control measures which include the judicious use of antibiotics, antibiotic cycling, the implementation of appropriate infection control measures and the formulation of an antibiotic policy must be done, to prevent the spread of these strains. Keywords: Pseudomonas aeruginosa, Acinetobacter baumannii, Antibiogram