Abstract
Economical and eco-friendly lignocellulose pretreatment technology, and enzymatic sugar production are an urgent demands for fermentation into valuable platform chemicals. In this work, we explored that pretreatment of corn cobs and corn stalks based on scCO2 at pressure (35–45 MPa), temperature (40 ℃−70 ℃) and moisture content (0 wt% − 85 wt%), time (1–24 h), and in term of pretreatment effect to investigate the enzymatic hydrolysis yield. The pressurized biphasic system of CO2-H2O generates carbonic acid in situ to promote the hydrolysis of biomass, and the drastic release of enclosed static scCO2 creates irreversible structural fragmentation for the two raw materials, as supported by various characterization techniques. Additionally, comparing the divergences in sugar yield and pretreatment mechanism between the two raw materials was due to the dominant tissue structure. Whilst trade-off high CO2 pressure and mild pretreatment temperature could achieve high enzyme digestibility compared to the untreated biomass.’
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