Abstract
BACKGROUND: Preservation injury is a phenomenon adversely affecting the quality of transplant grafts. When attempting to identify its degree, suitable parameters are required. In an effort to find a feasible and clinically applicable parameter, we aim to evaluate the usability of IL-2 as a parameter to evaluate quantitatively preservation graft injury. METHODS: IL-2 levels in preservation solutions effluent including Histidin-Tryptophan-Ketoglutarate (HTK) and University of Wisconsin (UW) solutions used in rat (Wistar strain) small bowel graft preservation were determined using ELISA and Quantikine M rat IL-2 R2000. We investigated the effect of UW and HTK solutions on preservation injury of rat jejunal grafts during 0-, 1-, 6-, 9-, and 12-hour cold ischemia time (CIT). RESULTS: In spite of the presence of upwards trend line proportionate with the increase in CIT, these cumulative changes in IL-2 levels are not statistically significant and might not be specific. Although HTK effluent contained higher levels of IL-2 at 0, 6, and 12 hours CIT than UW effluent, these differences are statistically insignificant (p>0.05) CONCLUSIONS: While the outcome of this study is negative, it shows that IL-2 is not a suitable marker to determine the degree of small bowel graft preservation injury in rats.
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