Abstract
The disaccharide 1-O-α- d-glucopyranosyl- d-fructose (trehalulose) was obtained in a 98% pure form, and in good yield by preparative-scale high-performance liquid chromatography (HPLC). Purification was from a complex mixture containing a trisaccharide, sucrose, isomaltulose, glucose and fructose, produced by immobilised microbial cells. Four reversed-phase preparative-scale HPLC columns were used in series with distilled water as the eluent. The structure of trehalulose was shown to be an equilibrium mixture of 1-O-α- d-glucopyranosyl-β- d-fructopyranose (66%) and 1-O-α- d-glucopyranosyl-β- d-fructofuranose (33%) by 13C NMR spectroscopy and other methods. This work demonstrates how gram quantities of pure material can be conveniently obtained using preparative HPLC, even when the components in a mixture are difficult to resolve.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
