Abstract
A mixture of oligogalacturonic acids was prepared by partial enzymatic hydrolysis of citrus pectin-derived α- d-polygalacturonic acid. Both a commercial fungal “pectinase” preparation and a purified endo-polygalacturonase isolated from this source by chromatography on carboxymethyl Sephadex C-50 were used to catalyze the hydrolysis. Different product distributions resulted, but even when using pectinase there was no evidence for the formation of unsaturated products from activity of polygalacturonic acid lyase. Individual oligogalacturonic acids were isolated by step-gradient elution (sodium formate, pH 4.7) from the macroporous strong base anion-exchange resin AG MP-1 in the formate form. Pure oligomers to heptagalacturonic acid were isolated in a single run, including gram quantities of tri-, tetra-, and pentagalacturonic acid. The individual oligogalacturonic acids were characterized by fast-atom bombardment mass spectrometry in positive and negative modes.
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