Abstract
A rapid method of microwave-assisted extraction coupled with high-speed counter current chromatography and UV detector was established for the preparation of phytosterols from edible marine algae. Extraction conditions, namely microwave power, liquid/solid ratio, irradiation time and extraction temperature were optimized using orthogonal array design. The microwave-assisted extraction of Undaria pinnatifida and Sargassum fusiforme were separated and purified with a non-aqueous two-phase solvent system composed of n-hexane–acetonitrile–methanol (5:5:3, v/v/v). The isolation was done in less than 220min, and a total of 13.0mg fucosterol, 1.5mg 24-methylenecholesterol and 10.7mg phytol were obtained from 15.0g U. pinnatifida; 4.6mg fucosterol, 0.3mg 24-methylenecholesterol and 3.5mg phytol were obtained from 15.0g S. fusiforme. The purities of all products were over 97% determined by high performance liquid chromatography. The results demonstrate that microwave-assisted extraction coupled with high-speed counter-current chromatography is a feasible, economical and efficient technique for rapid extraction, separation and purification of effective compounds from natural products.
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