Abstract
Synthesis of two lignin derivatives, lignophenol and lignin-aminophenol, were presented in this article. The chemical structure and the func-tional groups of lignin derivatives were charac-terized through FT-IR analysis. The immobiliza-tion of three proteases (papain, trypsin and pepsin) on lignin and lignin derivatives was carried out using adsorption technique. The influence of contact time and pH on the enzyme adsorption by different adsorbents was inves-tigated. Furthermore, enzyme activity recovery was also evaluated. Results showed that lignin and lignin derivatives could adsorb proteases effectively and the adsorption capacity of lingo- phenol and lignin-aminophenol was higher than that of pure lignin. Meanwhile, the activity re-covery of papain and pepsin immobilized on lignin derivatives was very high. This pheno- menon suggested that there is a supramole- cular interaction between enzymes and lignin derivatives which do not inhibit enzyme activity. Therefore, lignophenol and lignin-aminophenol are both promising adsorbents for enzyme im-mobilization under acid and neutral conditions.
Highlights
Trypsin and pepsin are all of proteases found in plant and animal cells that break down proteins or peptides by catalyzing the hydrolysis of peptide bonds
The results received from adsorption experiments show that three proteases can be adsorbed by lignin and lignin derivatives effectively
The adsorption capacity was affected by contact time and pH depending on the feature of enzymes
Summary
Trypsin and pepsin are all of proteases found in plant and animal cells that break down proteins or peptides by catalyzing the hydrolysis of peptide bonds. Lignin (EH-lignin) isolated from the residue of enzymatically hydrolyzed cornstalks (a by-product of fuel ethanol industry) is a novel organosolv lignin developed in recent years [7]. Making use of this biomaterial will enhance economic benefits of bioindustry and diminish potential environmental pollutions. Due to the unique isolation procedures, the content of polar functional groups, such as carbonyl, hydroxyl and phenolic hydroxyl, is very high in EH-lignin This characteristic makes it possible to utilize EH-lignin, after mild modification, as enzyme immobilization supports. The influence of contact time and pH on the enzyme adsorption was discussed in detail
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