Abstract
Preparing leaf anatomy slide is a routine procedure in physiology and anatomy plant research. A standard microtechnique method frequently adopted for doing such procedure is Johansens’. However, for early user such as university students, executing this method may be challenging in term of using many chemicals in different stages which make it more pricey and need longer duration. This research attempts to analyse the possibility for using simpler protocol suggested by Gunarso to replace Johansens method. It is conducted under a qualitative experiment design involving leaves from monocotyl and dicotyl classes as samples. Samples are analysed with safranin-fast green staining protocols. Result shows that there is no difference between Johanssens’ and Gunarso modified protocol (GMP) with respect to vivid observable of the cell membrane, the tissue structure, and the colour intensity. Hence, this new modification protocol is potential to replace the Johansens methods in leaf anatomy slide
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callMore From: IOP Conference Series: Earth and Environmental Science
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
