Abstract
Inverse suspension combined with the sol-gel method was deployed to prepare a magnetic chitosan microspheres.The lipase was immobilized on magnetic chitosan microspheres with genipin as the cross-linking reagent.The optimum conditions and properties of immobilization enzyme were investigated.The maximal specific activity of 4.31 U/g was achieved when the crosslinking reaction was carried out at 55 ℃ for 8 h using 0.6 g/L genipin.The optimum pH,temperature and Km of the immobilized enzyme were 8,25～35℃ and 0.26 mol/L,respectively.The immobilized lipase showed improved thermal stability and remained active in a broader pH range,compared with those of free lipase.Such immobilized lipase can be recycled via the magnetic separation for a certain extent.
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