Abstract
Objective: In this study, β-cyclodextrin (β-CD) was chosen as the coating for ellagic acid to prepare ellagic acid microspheres, and the effect of microspheres on the growth of HepG2 cells was observed. Methods: Scanning electron microscopy, infrared spectroscopy, and release rate analysis were used to identify the formation of ellagic acid microspheres. Methyl thiazolyl tetrazolium (MTT) assay was used to detect the effect of different concentrations of ellagic acid microspheres on tumor cell proliferation at 6, 12, 24 and 36 h, and cell morphology and quantity were observed using hematoxylin-eosin (HE) staining. Single-cell gel electrophoresis was used to observe the effect of ellagic acid microspheres on the DNA damage of HepG2 cells, and the Olive tail moment and the mRNA expression of tumor suppressor protein gene p53 was measured. Results: β-CD could be used as wrapping material of ellagic acid to prepare ellagic acid microspheres. HepG2 cell proliferation could be inhibited by 0.1, 0.3 and 0.5 g/L of ellagic acid microspheres in a dose- and time-dependent manner, and the mechanism of proliferation inhibition was related to DNA damage and cell apoptosis. Conclusion: Preparing ellagic acid microspheres with β-CD is feasible, and ellagic acid microspheres have potential therapeutic value (anticancer).
Highlights
Pomegranate (Punica granatum L.) belongs to the Punicaceae family and originates in CentralAsia [1]
The pomegranate peel ellagic acid (Figure 1B) was a needle-shaped indicated by the arrows (n = 20)
Needle-like ellagic acids were dispersed on the surface or interior spherical of the particles, it was and speculated that β-CD-ellagic acid microspheres formed. formed
Summary
Pomegranate (Punica granatum L.) belongs to the Punicaceae family and originates in CentralAsia [1]. Pomegranate (Punica granatum L.) belongs to the Punicaceae family and originates in Central. Pomegranates are widely grown in China, and the famous production areas include Zaozhuang in Shandong province, Mengzi in Yunnan province, Lintong in Shaanxi province, Kaifeng in Henan province, Huaiyuan in Anhui province, Yecheng in Xinjiang province, and Huili in Sichuan province [2,3]. Ellagic acid is a polyphenol and a dimeric derivative of gallic acid. It shows the tannin structure of trans gallic acid and has antioxidant, antibacterial, and antitumor properties, as well as other biological activities [6,7,8]. Researchers widely studied the inhibition of ellagic acid in cancer induced by chemicals
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