Abstract
Aim:The present work aimed to develop lateral flow immunochromatographic strip (ICS) test for detection of Salmonella Enteritidis (SE) specific antibodies in chicken sera.Materials and Methods:A rapid lateral flow immunochromatographic test (LFIT) has been developed, in which SE Group D antigen labeled with the gold chloride molecules laid on the conjugate pad. Staphylococcus aureus protein A was used as capture antibody at the test line (T) of a nitrocellulose (NC) membrane and anti-SE antigen-specific rabbit antibodies were used as capture antibody at the control line (C) of the NC strip in the lateral flow layout device.Results:Using the developed LFIT, the minimal amount of SE-specific antibodies that can be detected in chicken serum sample was 1427 enzyme-linked immunosorbent assay (ELISA) unit/100 µl that was equal to 0.1 µg (Ab)/100 µl sample. 100 suspected serum samples collected from a poultry flock were tested with the prepared SE-LFIT kits and the locally prepared stained Salmonella antigen, and the results were compared with those obtained from examination of these samples with Salmonella Group D antibody ELISA kit as the gold standard test. The sensitivity, specificity, and accuracy of the prepared SE-LFIT antigen kits were 94.4%, 90%, and 94%, respectively, while those obtained with stained Salmonella antigen were 88.8%, 90%, and 89%, respectively.Conclusion:The developed test is a simple field rapid test of high sensitivity, specificity, and accuracy that can improve and facilitates rapid field surveillance of salmonellosis among chickens.
Highlights
Salmonella spp. are among the most important agents of foodborne diseases worldwide
Human Salmonella outbreaks are often associated with the consumption of poultry products, and one of the most prevalent serotypes associated with these products is Salmonella Enteritidis (SE) [1]
Control of Salmonella infections in poultry is posing itself as one of the difficult problems for those who are concerned with poultry industry and for public health hazard because most of the serovars of Salmonellae which poultry harbor can act as potential pathogens for man [3]
Summary
Poultry may carry some Salmonella serovars without any signs or symptoms of disease and without causing any adverse effects to the health of the bird [2]. Control of Salmonella infections in poultry is posing itself as one of the difficult problems for those who are concerned with poultry industry and for public health hazard because most of the serovars of Salmonellae which poultry harbor can act as potential pathogens for man [3]. The Creative Commons Public Domain Dedication waiver (http:// creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated
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