Abstract
A simple way was proposed to prepare reverse phase open tubular column (OTC) for capillary electrochromatography (CEC) by dynamically coating cationic latex particles with C10 functional groups onto the fused silica capillary with surface sulphonation. The latex particles are bounded into the capillary wall via electrostatic attraction force and demonstrate good stability under test conditions. Relative bare silica capillary, reverse electroosmotic flow (EOF) is generated for the OTC due to the existence of cationic latex particles onto the capillary. Preliminary separation of aromatic compounds on an OTC with 50 i.d. has been demonstrated.
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