Abstract
ObjectiveTo study the characterization of Fe3O4@Au-C225 composite targeted MNPs.MethodsFe3O4@Au-C225 was prepared by the absorption method. The immunosorbent assay was used to evaluate its absorption efficiency at C225 Fc. ZETA SIZER3000 laser particle size analyzer, ultraviolet photometer and its characteristics were analyzed by VSM. the targeting effect of Fe3O4@Au-C225 composite targeted MNPs on U251 cells in vitro were detected by 7.0 Tesla Micro-MR; and subcutaneous transplanted human glioma in nude mice were performed the targeting effect in vivo after tail vein injection of Fe3O4@Au-C225 composite targeted MNPs by MRI.ResultsThe self-prepared Fe3O4@Au composite MNPs can adsorb C225 with high efficiency of adsorption so that Fe3O4@Au-C225 composite targeted MNPs were prepared successfully. Fe3O4@Au-C225 composite targeted MNPs favorably targeted human glioma cell line U251 in vitro; Fe3O4@Au-C225 composite targeted MNPs have good targeting ability to xenografted glioma on nude mice in vivo, and can be traced by MRI.ConclusionThe Fe3O4@Au-C225 composite targeted MNPs have the potential to be used as a tracer for glioma in vivo.
Highlights
The primary malignant central nervous system tumor is among 1.49% of all cancer and the rate of disability and mortality is considerably high [1, 2]
Gold can mediate NIR thermotherapy as a sensitizer of light for hyperthermia depended electron-dense, dielectric property, high adsorption cross section and high Light-thermal conversion efficiency [6]. It can be used for fixing EGFR monoclonal antibody (McAb) cetuximab (C225), constructing new nanocomposite which gathers molecular targeted therapy, MRI, MFH and NIR thermotherapy by preparing Fe3O4@Au composite magnetic nanoparticles (MNPs)
The preparation and application of Fe3O4@Au composite MNPs have been reported before the present study, but the research of it used as a carrier to fix commercially available EGFR McAb-C225 for anti-glioma has not been conducted before
Summary
The primary malignant central nervous system tumor is among 1.49% of all cancer and the rate of disability and mortality is considerably high [1, 2]. The nanoscale device, with merely 1~100nm in its length, can enter and leave human cells freely It has the advantages of less volume, better biocompatibility, and superior targeting ability to specific tissus/cells compared to traditional tracers. Gold can mediate NIR thermotherapy as a sensitizer of light for hyperthermia depended electron-dense, dielectric property, high adsorption cross section and high Light-thermal conversion efficiency [6] It can be used for fixing EGFR monoclonal antibody (McAb) cetuximab (C225), constructing new nanocomposite which gathers molecular targeted therapy, MRI, MFH and NIR thermotherapy by preparing Fe3O4@Au composite MNPs. The preparation and application of Fe3O4@Au composite MNPs have been reported before the present study, but the research of it used as a carrier to fix commercially available EGFR McAb-C225 for anti-glioma has not been conducted before. This article uses the method of MRI to evaluate the targeting ability of the self-prepared Fe3O4@AuC225 composite targeted MNPs in vivo and in vitro
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
